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Chronic HFD DIO studies were limited to male mice, as female mice display attenuated weight gain 28,29

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performed an experimental study and determined PS exposure, RBC volume, cytosolic Ca 2+ activity, and ceramide formation (flow cytometer evaluation for all parameters)

Animal ethics statement All procedures, protocols, and medications were approved by the Johns Hopkins University Animal Care and Use Committee and were performed in accordance with the Association for Research in Vision and Ophthalmology statement for use of animals in ophthalmic and vision research, and the National Institutes of Health guide for the care and use of laboratory animals

Patients using multi-dose vials should always use a new syringe and needle for each injection