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(C) IH growth to adjacent cells was significantly reduced (Students t-test p0.05) in gtr1 strains compared to WT at 48 hpi

Three clones were picked and transferred into 5 mL LB liquid medium supplemented with 100 M ampicillin sodium (Amp) and 50 M spectinomycin dihydrochloride pentahydrate (Spe), and incubated at 37 C, 220 rpm for 6 h in an orbital shaking incubator

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