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GST pull-down assays For GST pull-down assay, a tube of the 1 ml mouse liver cytosol was thawed on ice, centrifuged at 20,000 x g for 20 minutes at 4 C to remove any precipitated material, and the clarified cytosol was then diluted to a total protein concentration of approximately 20 mg/ml with cold homogenization buffer B (homogenization buffer A containing TX-100 at a final concentration of 0.1%)

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