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Material and methods Cell culture Human hepatocellular carcinoma (HepG2) (ATCC, HB-8065) were maintained in DMEM (4.5 g/l glucose, pyruvate) (Gibco), supplemented with 10% fetal bovine serum (PAA Laboratories) and penicillin/streptomycin (25 g/ml each, Gibco) at 37 C, 5% CO 2

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The company also offers Tirzepatide (a dual GLP-1/GIP agonist showing 2022% weight loss in trials) or the GLP-Squared dual-compound injection protocol for patients who may benefit from synergistic receptor activationall without added stacking risk

doi: 10.1073/pnas.1912129116, 93 SongD.LiH.LiH.SongD.LiH.DaiJ

These diseases significantly increase the risk of other severe health complications like cardiovascular disease, chronic kidney disease, and even death

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