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Nature 385: 214, 1997 [DOI] [PubMed] [Google Scholar] 45

rhodozyma cells was conducted through the electro-transformation method with slight modifications, as described by Niklitschek et al

Wolf Horrell EM, Boulanger MC, D'Orazio JA

Free PCSK9 in standards, controls and samples was captured by a biotinylated monoclonal anti-human PCSK9 antibody at 100 g/mL (in-house) and detected by an Alexa-labelled PCSK9 Affinity Purified PAb antibody at 30 nM (R&D Systems) on a Gyrolab Bioaffy 1000 CD platform (Gyros AB)