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Is CREB a key to neuronal survival

Freezing causes ice crystal formation that physically disrupts the peptides molecular structure

Methods: We first optimized the concentration of MARE that could be used to treat human dermal fibroblasts (HDFs) without causing cytotoxicity

Cultures were maintained in a humidified atmosphere of 5% CO 2 at 37C for 24 h and then incubated for the next 24-h period in DMEM-10% HS additionally containing recombinant HGF that was treated with peroxynitrite with or without trisulfide pre-treatment (see Figure 1d,f)

If still well-tolerated after another 2 weeks, move to nightly

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