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Denatured proteins are precipitated by cooling and removed via centrifugation, keeping of NAD + , NADH, NADP + , NADPH, GSSG and GSH stable in the supernatant

Fully differentiated HepaRG cells (Lonza, catalog: NSHPRG) were plated with 90% of Williams E Medium (Thermo Fisher Scientific, catalog: A1217601) and supplemented with 10% HepaRG thawing and plating medium supplement (Lonza, catalog: MHTAP) and 25 U/mL penicillin-25 g/mL streptomycin

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This direct activation of a critical angiogenic pathway, independent of VEGF-A expression, highlights a unique aspect of BPC-157's regenerative capacity