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The general approach for inhibitor identification is outlined in Fig

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The interacting proteins can then be eluted and analyzed, typically through SDS-PAGE followed by mass spectrometry or Western blotting, to identify and characterize the protein interactions

ScGrx7 and PfGrx both have typical ping-pong kinetics with GSSCys and GSH and cannot be saturated at infinite substrate concentrations in accordance with previous results from GSSEtOH assays on ScGrx7 (ref

Most published data derive from small pilot studies, in vitro experiments, and animal research, which substantially limits the conclusions that can be drawn