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Analysis of Intracellular and Extracellular Low Molecular Mass Thiols (LMM-SH) In those experiments where the intracellular levels of GSH, NAC and NACET were measured, the culture medium was removed and the cells were washed twice (1 min each) with phosphate-buffered saline solution (PBS), pH 7.4, at 4 C, lysed by treatment with 0.5 mL of a 4% ( w / v ) solution of ice-cold TCA containing 1mM K3EDTA and collected after scraping