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Proximal tubular epithelial cells reabsorb urate through coordinated transport mediated by URAT1 (SLC22A12) and GLUT9 (SLC2A9)

The erythrocytes were then lysed with lysis buffer (BD Pharm Lyse, BD Bioscience, San Jose, USA) for 10 min followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and resuspension of the cells in Hepes-buffered saline (HBS, 14 mM Hepes, 0.9% NaCl)

For others, evening injection timing avoids practical issues

She resides in Phoenix with her wife and twin daughters

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This minimizes competition with other nutrients and supports better absorption, though liposomal delivery methods offer more flexibility