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Immunofluorescence staining For immunofluorescence, cells were seeded on the Glass Bottom Cell Culture Dish (NEST)

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201,202 Activation of collagen synthesis Collagen, the most abundant protein in the body, becomes a crucial part of the ECM, and the biological adhesive that holds cells together

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Outcome measures include biomechanical tensile strength testing of repaired tissue, histological scoring of collagen fiber organization, and immunohistochemical staining for fibroblast and endothelial cell markers