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Cell were then treated with Cy5.5-labeled NP4 for 1 h, 4 h, or 7 h, respectively, washed with PBS, and analyzed by flow cytometry (Beckman Coulter, U.S.A.)

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They can degrade, clump, or lose their effectiveness if not stored and handled properly

Cobalamin is separated from IF by lysosomal enzymes and released into the circulation for transport to target tissues [26]