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Research published in the Journal of Clinical Investigation Insight confirmed that this PHB-ANXA2 complex exists in both mouse and human white fat vasculature, which was a critical finding for translational relevance

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UBE2D2 and Ub were isolated using cation-exchange chromatography and eluted with either 50 mM HEPES pH 7, 250 mM NaCl, 5 mM DTT or 25 mM sodium acetate pH 4.5, 250 mM NaCl, respectively

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