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The supernatants were centrifuged at 10,000 g for 15 min at 4C, and the mitochondrial pellets were dissolved in sodium dodecyl sulfate (SDS) sample buffer, subjected to 15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE), and analyzed by immunoblotting with monoclonal antibodies against cytochrome c and Cox IV
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The results showed that LC significantly increased the expression of CYP11A1(cytochrome p450 11A1) (related to steroidogenesis) and TXNIP (thioredoxin interacting protein) (related to oxidative stress) and increased serum levels of follicle-stimulating hormone (FSH), reduced testosterone, luteinizing hormone (LH), glucose, insulin, and homeostasis model assessmentinsulin resistance
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