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Plates were incubated at RT for 1 h and the fluorescence intensity measured at 620 nm and 650 nm by an EnVision multilabel plate reader (PerkinElmer)

If you miss a dose, the missed dose should be skipped, and the next dose should be taken the following day

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This forebrain structure is neuroanatomically interconnected with several regions before mentioned, such as the PBN, VTA, NAc, or amygdala [256]