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Inactive digestive enzymes are packaged into condensing vacuoles by the Golgi apparatus and stored in ZGs at the apical pole of acinar cells
FACS trafficking MIN6B1-SNAP-GLP-1R or CHO-SNAP-GLP-1R cells were pre-incubated in HEPES-bicarbonate buffer plus 3 mM glucose and 1% BSA before labeling at 37 C with 1 M cleavable BG-SS-488 SNAP-Surface probe (a gift from New England Biolabs) and stimulation with 100 nM agonist in HEPES-bicarbonate buffer plus 11 mM glucose and 1% BSA, and trafficking assays performed as follows 27 : Briefly, for receptor internalization, cells were placed at 4 C to arrest endocytosis following incubation at 37 C for the indicated times, or directly put at 4 C for 0 min time-point, and treated with ice-cold alkaline TNE buffer 100 mM MesNa to strip surface-exposed label (plus MesNa) or to measure total labeled receptor (minus MesNa)
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And supported by the program for Henan Engineering Technology Research Center of Food Processing, Circulation Safety Control (20171215), Henan Province Postgraduate Joint Training Base Project (YJS2022JD16), and Innovative Research Team (in Science and Technology) in University of Henan Province (No
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