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Storage and Handling Protocols Proper peptide storage significantly impacts protocol effectiveness
Thiol specific and mitochondria selective fluorogenic benzofurazan sulfide for live cell nonprotein thiol imaging and quantification in mitochondria
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The cell pellets were thawed and lysed in a buffer containing 20 mM HEPES, pH 7.5, 100 mM NaCl, 10% (v/v) glycerol, 10 mM MgCl 2 , 1 mM MnCl 2 , and 100 M TCEP supplemented with EDTA-free protease inhibitor cocktail (Bimake) by Dounce homogenization
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Umeda R, Teranishi H, Hada K, Shimizu N, Shiraishi H, Urushibata H, et al
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