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The pellet was once more washed with cytoplasmic lysis buffer and once with cytoplasmic lysis buffer without detergent

For the tumour allograft model, Gclc f/f Rosa26 creERT2 MMTV-PYMT mice were euthanized and autochthonous tumours were collected, cut into 8 mm 3 fragments and cryopreserved in liquid nitrogen

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The excess of ROS leads to increased expression levels of apoptosis-promoting factors p53, Cytc, Bax, and Caspase3

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