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This short, pulsatile pattern is characteristic of the compound

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The cells were lysed in lysis buffer [10 mM Tris (pH 7.4), 10 mM KCl, 2 mM MgCl, 1 mM EGTA, 1 mM DTT, 0.05% (v/v) Triton X-100 and 1 protease inhibitor (Pierce)] for 30 min on ice

2005;31(7 Pt 2):860-865