CDNB enzymatic assay GST activity was measured using 1-chloro-2,4-dinitro-benzene (CDNB, Sigma-Aldrich) as the substrate
Western blotting Samples were lysed in RIPA buffer containing a protease inhibitor, and the total protein content was quantified using a bicinchoninic acid protein assay kit (Beyotime)
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Cruciferous vegetables include Brussels sprouts, broccoli, cauliflower, kale, and mustard greens
Effects of L-carnitine and its derivatives on postischemic cardiac function, ventricular fibrillation and necrotic and apoptotic cardiomyocyte death in isolated rat hearts