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It is worth noting that HDACs can regulate the related signaling pathways such as phosphatidylinositol-3-kinase (PI3K)/protein kinase B (PKB/Akt) by affecting protein acetylation levels

doi: 10.13422/j.carol.carroll.nki.syfjx.20230941 186 WeiHZhaoRLiSDuoHHuoH

Therefore, relatively high laser power is required for confocal imaging, which can consequently cause phototoxicity to live cells, especially with excitation at 405 nm (ref