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As described within the experimental section, the GSH was denatured utilizing a heat bath at 70 C, mixed with the gold nanoparticle solution and 100 uL of each respective APTES concentration

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J Clin Endocrinol Metab 97:21242133 Haussler MR, Whitfield GK, Kaneko I, Haussler CA, Hsieh D, Hsieh JC, Jurutka PW (2013) Molecular mechanisms of vitamin D action

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