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Over-lap PCR was used to generate six GSTP phosphorylation-mimicking mutants ( Co-Immunoprecipitation and Immunoblotting Analysis Cells were washed twice with phosphate-buffered saline (PBS), and then lysed in a lysis buffer on ice for 30 min, and then cell lysates were centrifuged at 12,500 rpm for 15 min

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doi: 10.1016/j.nut.2004.04.014