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Briggs GG, Ambrose PJ, Nageotte MP, et al

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MGF is a splice variant called IGF-1Ec - same N-terminal sequence as IGF-1 but a different C-terminal E-domain that gives it distinct, locally acting properties

To investigate this, we inserted phosphorylated CAF-1 into yeast cells and observed the effects on gene expression

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The amount lost is typically negligible, but proper technique prevents it entirely