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Samples were permeabilized with 0.2% Triton X-100 (Sigma Aldrich) in PBS for 5 min at RT, washed with PBS and blocked with 2% BSA (Sigma Aldrich) in PBS for 30 min at RT to block non-specific binding sites cells

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In 2013, Bauer and Kriebel reported that prenatal use of acetaminophen was strongly correlated with autism/Autism Spectrum Disorder prevalence ( r = 0.80) using all available country-level data ( n = 8) for the period 1984 to 2005