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First, TAZ interacts with RORt to increase its transcriptional activity while competitively relieving Foxp3-mediated repression, thereby coordinately upregulating Th17-associated gene expression and promoting Th17 cell development

On the day of the experiment, wells were washed twice with 500 l Ca 2+ -free buffer containing (mmol/l): 135 NaCl, 5 KCl, 2 NaHCO 3 , 0.5 NaH 2 PO 4 , 0.5 MgCl 2 , 0.4 EGTA, 10 HEPES (pH 7.4 with NaOH)

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Underwood WJ, McGlone JJ, Swanson J, Anderson KA, Anthony R

2014;72 Suppl 1:20-25