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Hippocampal neurons were then cultured a further 3-7 days, at which time they were either transfected or treated with various pharmacological reagents as described in (Wayman et al., 2008)
In protein-calorie malnutrition, both GSH and CYP2E1 quantities would be reduced (as detailed by a reduction in both GSH and CYP2E1 messenger RNA) and thus there would be no net overall effect on toxicity [16, 85]
Although structural insights into other FBXO22 native substrates are limited, our biochemical and cell-based data further support the notion that recruiting neosubstrates to the FBXO22 surface via degraders that engage Cys326 is unlikely to significantly perturb native substrate degradation
Frana BK, Alves MRM, Souto FMS, et al
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