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Sample preparation and extraction Plasma samples were thawed at 4 C and a volume of 200 L of each sample was transferred into a vial and extracted with 600 L of methanol

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Funding This work was supported by the CNPq/Brazil, Bill & Melinda Gates Foundation (process 401726/2015-0 APP/Call 47/2014) and the INCT/Excitotoxicity and Neuroprotection (465671/2014-4)