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Direct Sequence Analysis of a Portion of the NS5B Gene Followed by Phylogenetic Analysis (Reference Method) Briefly, total RNA was extracted from 200 l of serum by using the High Pure Viral RNA kit (Roche Molecular Biochemicals, Mannheim, Germany), according to the manufacturer's instructions

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The latter is also known as the pineal gland, which produces melatonin and regulates the sleep-wakefulness cycle