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doi: 10.1111/imr.12160 39 YueXTrifariSijTTsagaratouAPastorWAZepeda-MartnezJAet al

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Phase 4: Maintenance optimization (weeks 25 onward) This is where individual adjustment matters most

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After 15 min of incubation at room temperature in the dark, cells were washed, resuspended in the binding buffer, and DAPI was added immediately before analysis on a Fortessa X-20 Cell Analyzer (BD Biosciences)