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The dissected brains were incubated in 100 nM TMRE solution (Thermo Fisher Scientific) in DSM for 10 min and relative fluorescence intensity was quantified by top excite-read method through Synergy H1 reader

The peptide was allowed to denature over a 4 hour time period and 100 uL aliquots of the peptide solution was removed, mixed according to the above mentioned procedure and characterized

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