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For Tirzepatide, there is no established washout time in the datasheet

Unlike regular IGF-1, IGF-1 LR3 has an extended half-life, which prevents rapid degradation and allows it to remain active in the body longer (4)

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Senescent Leydig Cell Elimination & Testosterone Restoration in Aged Mice (Aging / PMC, 2020) FOXO4-DRI Disrupts FOXO4-p53 in Senescent Leydig Cells and Improves Testicular Microenvironment A study published in Aging (and indexed on PMC) demonstrated that FOXO4 is specifically expressed in human Leydig cells, with nuclear translocation of FOXO4 in elderly men correlating with decreased testosterone synthesis

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The link between weight loss and increased bone resorption markers is supported by several studies in the literature [29]