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2.5 Collection of tissue samples and histometric measurements On day 138 of the study, the animals were slaughtered at a local slaughterhouse, and the ovarian tissue was promptly placed in 10 % formalin solution

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The isolated TIF was snap-frozen in liquid nitrogen and stored at 80 C before analysis

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30 min later, the cells were photographed with a CLSM

45 Specifically in patients with pancreatic cancer, high doses of vitamin C have been shown to impede the growth of pancreatic ductal adenocarcinoma cells through reduction in glucose metabolism, trigger apoptosis, and suppress invasion and metastasis of pancreatic adenocarcinoma cells