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Nemkov, T., Hansen, K

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These proteins are located on the IMM and interact with the optic atrophy protein 1 (Opa1)

A florescent probe like 2,7-dichlorofluorescein diacetate (H 2 DCFDA) penetrates the spermatozoa and indicates H 2 O 2 concentrations, as H 2 O 2 de-esterifies in the presence of DCFH and forms fluorescent 2,7-dichlorofluorescein (DCF) ( Dihydroethidium/hydroethidine (DHE) is a non-florescent probe that is oxidized by a variety of reactive oxygen and nitrogen species