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Differentiated cells were incubated in media with 1 mg/ml ICG for 1 hour at 37 C, washed with PBS, and imaged using a phase-contrast microscope

A compound can show clean short-term animal data and still present unforeseen problems in chronic human use this is a well-documented pattern in drug development history

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Unlike cloudiness caused by air bubbles, which often subsides in minutes or seconds, sediment will always make your water look cloudy