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In the pregnant rat (30 rats per group), twice-daily exposure to an aerosol of acetylcysteine and isoproterenol for 30 or 35 minutes from the 15th day of gestation through the 21st day postpartum was without adverse effect on dams or newborns

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Starting with lower doses and monitoring for adverse effects is a standard, cautious approach before adjusting upward based on clinical response

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Recombinant proteins were analyzed using 10% SDS-PAGE and purified using HisTrap HP Crude Columns (GE Health) and AmiconUltra-15 Centrifugal Filter Units with Ultracel-3 membranes (Merck Millipore)