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Curr Opin Biotechnol 22:300307 Xu L, Eu JP, Meissner G, Stamler JS (1998) Activation of the cardiac calcium release channel (ryanodine receptor) by poly-S-nitrosylation

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Samples were then precipitated with 1 mL absolute MeOH at 40 C for 2 h, centrifuged (16,000 g ), and the obtained supernatants were evaporated, and the residue was resuspended and sonicated in 100 L 50 mM HEPES buffer (pH 7.4)

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