Images were obtained at 20 magnification using a light microscope (Leica DM LB) and digitally photographed for automated image analysis
After 4 h incubation at room temperature, the sample concentration in the donor and acceptor wells were measured using a UV-Vis spectrophotometer (Beckman, DU640) set at the wavelength corresponding to the maximum absorption of each compound
Briefly, 1 mM stock solution of each compound was prepared in deionized water, diluted to a final concentration of 400 M in PBS (Sigma Aldrich
5-Amino-1MQ dosage protocols center on this selective, cell-permeable NNMT (Nicotinamide N-methyltransferase) inhibitor studied for its potential to support fat metabolism, preserve lean muscle mass, and elevate intracellular NAD+ levels [1] [2]
Individual results vary and should be discussed with your physician
Choice of inhibitor concentrations and time period was chosen based on the results from the MTT studies