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Luminescence signals (Alpha photon counts) were measured using an EnSpire Alpha microplate reader (Revvity) according to the manufacturers instructions

Alpha lipoic acid is another important antioxidant that increases glutathione levels in a couple of ways: it is able to regenerate oxidized (i.e., used) glutathione back to its usable form, in addition to providing a continuous source of cysteine and increasing the uptake of cysteine into cells, where it increases glutathione production within the cell

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Those neurons don't turn over