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After extraction with 340 l of chloroform and 340 l of H 2 O, the upper aqueous phase was applied to DOWEX AG-1 columns

We gradually increase the dosage over time to allow the body to adjust and minimize potential gastrointestinal side effects

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Furthermore, the minimization of overall solvent and reagents needed can help overcome cost barriers to encourage the use of greener solvents and reagents that are highly desirable, but more expensive