Control groups of mice were maintained on LD or WD and given sham (10 mL/kg body weight sterile saline) injections throughout the study (termed LD/LD-V, or LD control, and WD/WD-V, or WD control, groups
Empty Sterile Glass Vials Empty vials are sterile, nonpyrogenic vials made with USP Type I glass that are used for mixing different solutions for administration
This bacteriostatic water is USP grade, pH-balanced, and preserved with 0.9% benzyl alcohol for multiple draws
Cell cycle analysis 5-Ethynyl-2-deoxyuridine (EdU) incorporation assay was performed by using Click-iT Plus EdU Flow Cytometry Assay Kits (Thermo Fisher Scientific Inc.) following the manufacturers protocol
TB-500 is systemically acting - distributes throughout the body via actin regulation, dosed in milligrams (25 mg), given twice weekly
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