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Theyre more likely to cause discomfort, inflammation, or even pain during or after the injection, which can make regular dosing unpleasant and less sustainable

For immunofluorescence staining, cells were rinsed three times with PBS before being incubated with BODIPY TM 581/591 C11 dye in serum-free medium at 37 C with 5% CO2 for 30 min before being visualized under a fluorescence microscope (Evos FL, Thermo Fischer Scientific) with objective 40

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