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After loading the supernatant, the column was washed with 20 column volumes of 90% buffer A (0.5 M NaCl, 0.2 M sodium phosphate, and 20 mM imidazole) and 10% buffer B (0.5 M NaCl, 0.2 M sodium phosphate, and 0.5 M imidazole) followed by elution with 100% buffer B

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Additionally, we will highlight the implications of these findings for natural medicine practice in general medical and identify gaps in knowledge that must be addressed to support its wider incorporation into integrative care

84 [online] Available at: Rajdl, D., Racek, J., Trefil, L., Stehlik, P., Dobra, J., and Babuska, V., (2016) Effect of folic acid, betaine, vitamin B 6 , and vitamin B 12 on homocysteine and dimethylglycine levels in middle-aged men drinking white wine, Nutrients, Vol