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Target gene expression levels were normalized by 18 S ribosomal RNA (18 S ribosomal N1), and relative gene expression was calculated using the 2e(-Ct) method 21

While TM is recognized as a pan-allergen, the presence of other allergens, such as arginine kinase (AK), sarcoplasmic calcium-binding protein, and hemocyanin, may influence individual clinical reactivity, as some patients may react solely to these allergens rather than the pan-allergen [46]

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