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Age, sex, BMI, diabetes status, genetic markers, microbiome composition, and lifestyle factors all influence outcomes

The cell pellets were thawed and lysed in a buffer containing 20 mM HEPES, pH 7.5, 100 mM NaCl, 10% (v/v) glycerol, 10 mM MgCl 2 , 1 mM MnCl 2 and 100 M TCEP (Sigma-Aldrich) supplemented with EDTA-free protease inhibitor cocktail (TragetMol) by dounce homogenization

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Kaufman 1994)

SAK increases the expression of antioxidant genes by activating the NRF2 signaling pathway