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The vial contains tirzepatide as the active peptide, cyanocobalamin (vitamin B12) typically at concentrations around 0.5-1mg per vial, and often glycine as a third component

If a third session is required, it will be scheduled 1 to 3 months later

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The time program for separation was as follows: initial incubation for 2.5 min at 0% solvent B followed by an increase in solvent B to 10% in 7.5 min and a final incubation for 7 min at 100% solvent B

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