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HC neutrophils were cultured in the presence of HC or SLE serum with or without the addition of Cl-amidine (Cl) (peptidyl arginine deiminase 4 (PAD4) inhibitor, 100 M), or LPX-1 (1 M) for 4 or 16 hours and NETs were assessed in SYTOX Green+ cells based on morphology (n = 6)

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For a - i , n = 3 biological independent experiments