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Following double digestion and purification, both natively paired and combinatorial scFv libraries were generated by ligating 5 g of the appropriate scFv insert into 25 g of the double-digested phagemid vector, LVEC2295 (proprietary to Sanofi), at a phagemid:insert ratio of 1:3 using T4 DNA Ligase (NEB, catalog no

Pediatric Radiology February 1997, Volume 27, Issue 2, pp 155-158 12 K

Despite achieving complete remission following a combination of subtractive surgery and first-line chemotherapy, a substantial proportion of patients (range, 70-80%) experience recurrence within 2-5 years

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Maintaining these benefits requires living a healthy lifestyle

Khan et al., 2012