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Spinning disk confocal microscopy For clear organoid visualization, we used the ultrafast confocal system IXplore SpinSR Olympus (Olympus, Tokyo, Japan)

The mechanism is better described as partially proposed than established

Plain sterile water is not preservative-free bacteriostatic once the vial seal is broken, it is susceptible to microbial contamination and should only be used for single-draw applications

doi: 10.1001/archneur.1991.00530200044017 185 RivestS