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In the context of ARDS, ferroptosis contributes to the destruction of alveolar and endothelial cells, leading to increased vascular permeability, pulmonary edema, and impaired gas exchange

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In brief, reagent 1 (pH 1.75) of TOS was prepared by adding 150 M xylenol orange, 140 mM NaCl and 1.35 M glycerol in 25mMsulphuric acid Reagent 2 was prepared by adding 5 mM ferrous ammonium sulphate and 10 mM o-dianisidine dihydrochloride

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